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ready-to-use 3d human hepatic stellate cell monoculture spheroids  (ScienCell)
90
ScienCell ready-to-use 3d human hepatic stellate cell monoculture spheroids
TGFβ induced expression of HSC activation markers in different 2D or <t>3D</t> culture conditions. ( a ) 3D culture of pHSC on a low attachment plate with constant shaking; ( b ) 3D culture of pHSC in high-viscosity media; ( c ) 3D culture of pHSC in Elplasia plate; ( d ) 2D culture of LX-2 cell© ( e ) 2D culture of pHSC from Donor 1; ( f ) 2D culture of pHSC from Donor 2. Cells were treated with 5 ng/mL of TGFβ for 24 h before collection for RNA isolation and RT-qPCR. For comparisons between two groups, a two-tailed, unpaired Student’s t -test was used. A p -value of more than 0.05 was considered non-significant (ns); a p -value between 0.01 and 0.05 was considered significant (*); a p -value between 0.001 and 0.01 was considered very significant (**); a p -value between 0.0001 and 0.001 was considered very extremely significant (***); a p -value of less than 0.0001 was considered extremely significant (****).
Ready To Use 3d Human Hepatic Stellate Cell Monoculture Spheroids, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+monocultures/pmc11206700-187-13-20?v=ScienCell
Average 90 stars, based on 1 article reviews
ready-to-use 3d human hepatic stellate cell monoculture spheroids - by Bioz Stars, 2026-08
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TGFβ induced expression of HSC activation markers in different 2D or 3D culture conditions. ( a ) 3D culture of pHSC on a low attachment plate with constant shaking; ( b ) 3D culture of pHSC in high-viscosity media; ( c ) 3D culture of pHSC in Elplasia plate; ( d ) 2D culture of LX-2 cell© ( e ) 2D culture of pHSC from Donor 1; ( f ) 2D culture of pHSC from Donor 2. Cells were treated with 5 ng/mL of TGFβ for 24 h before collection for RNA isolation and RT-qPCR. For comparisons between two groups, a two-tailed, unpaired Student’s t -test was used. A p -value of more than 0.05 was considered non-significant (ns); a p -value between 0.01 and 0.05 was considered significant (*); a p -value between 0.001 and 0.01 was considered very significant (**); a p -value between 0.0001 and 0.001 was considered very extremely significant (***); a p -value of less than 0.0001 was considered extremely significant (****).

Journal: Non-Coding RNA

Article Title: An Integrative Transcriptome Subtraction Strategy to Identify Human lncRNAs That Specifically Play a Role in Activation of Human Hepatic Stellate Cells

doi: 10.3390/ncrna10030034

Figure Lengend Snippet: TGFβ induced expression of HSC activation markers in different 2D or 3D culture conditions. ( a ) 3D culture of pHSC on a low attachment plate with constant shaking; ( b ) 3D culture of pHSC in high-viscosity media; ( c ) 3D culture of pHSC in Elplasia plate; ( d ) 2D culture of LX-2 cell© ( e ) 2D culture of pHSC from Donor 1; ( f ) 2D culture of pHSC from Donor 2. Cells were treated with 5 ng/mL of TGFβ for 24 h before collection for RNA isolation and RT-qPCR. For comparisons between two groups, a two-tailed, unpaired Student’s t -test was used. A p -value of more than 0.05 was considered non-significant (ns); a p -value between 0.01 and 0.05 was considered significant (*); a p -value between 0.001 and 0.01 was considered very significant (**); a p -value between 0.0001 and 0.001 was considered very extremely significant (***); a p -value of less than 0.0001 was considered extremely significant (****).

Article Snippet: For cells cultured in high-viscosity media, 3D spheroids were obtained from the Ready-to-use 3D Human Hepatic Stellate Cell Monoculture Spheroids (ScienCell Research Laboratories, Carlsbad, CA, USA), following the manufacturer’s instructions.

Techniques: Expressing, Activation Assay, Viscosity, Isolation, Quantitative RT-PCR, Two Tailed Test